Generator

Part:BBa_K2451002

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


MarA Generator

A production circuit for inducible expression of transcriptional regulator MarA. MarA is a transcriptional regulator in E. Coli responsible for regulating >60 chromosomal genes. It's primary purpose is to mediate expression of non-specific RND efflux pumps; conferring resistance to antibiotics and organic solvents through increased expression of AcrAB-TolC. MarA is a significant actor in the Mar-Sox-Rob regulon.



https://parts.igem.org/File:Benzaldehyde_Toxicity_Test.jpeg https://parts.igem.org/File:Benzaldehdye_Toxicity_Test_Controls.jpeg https://parts.igem.org/File:Ampicillin_Toxicity_Test.jpeg https://parts.igem.org/File:PMarA_Amp_Plates.jpeg https://parts.igem.org/File:PSB1C3_Amp_Plates.jpeg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 447
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 215
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None